Journal: Autophagy

Article Title: m 6 A mRNA methylation regulates testosterone synthesis through modulating autophagy in Leydig cells

doi: 10.1080/15548627.2020.1720431

Figure Lengend Snippet: Primary antibodies used in this study

Article Snippet: Cell Signaling Technology , Rabbit anti-PRKAA2 , 5831 , WB,1:1000.

Techniques:

Journal: Autophagy

Article Title: m 6 A mRNA methylation regulates testosterone synthesis through modulating autophagy in Leydig cells

doi: 10.1080/15548627.2020.1720431

Figure Lengend Snippet: Primary antibodies used in this study

Article Snippet: Cell Signaling Technology , Rabbit anti-p-PRKAA2 T172 , 50,081 , WB,1:1000 IF, 1:50.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Gleditsia sinensis Thorn Attenuates the Collagen-Based Migration of PC3 Prostate Cancer Cells through the Suppression of α2β1 Integrin Expression

doi: 10.3390/ijms17030328

Figure Lengend Snippet: WEGST treatment attenuated the cell adhesion and the expression of α2 integrin; the inhibitory effect of WEGST on PC3 cell migration was investigated. A representative figure of three independent experiments is exhibited in the upper panels. The number of attached cells was counted in four different fields and tabulated in the bottom panel ( A ); Semi-confluent PC3 cells were treated with WEGST; and the expression of α2 and β1 integrin was examined by western blotting ( B ); PC3 cells were pre-treated with WEGST and seeded on a collagen-coated plate. PC3 cells were harvested at various times, as indicated, and the expression of α2 and β1 integrin was examined by western blotting ( C ). The results shown are representative of at least three independent experiments. Error bars refer to the SD of the mean. Bar = 0.2 µm. * p < 0.05, compared with control (non-treated).

Article Snippet: The membranes were incubated separately with monoclonal anti-β1 integrin antibody (sc-374429; Santa Cruz, CA, USA), rabbit anti-α2 integrin antibody (Merck Millipore, Gibbstown, NJ, USA), and mouse monoclonal anti-β-actin antibody (Santa Cruz, CA, USA), followed by horseradish peroxidase (HRP)-conjugated anti-rabbit immunoglobulin G (IgG); enhanced chemiluminescence (ECL) reagents (Bio-Rad Co., Hercules, CA, USA) were used to detect signals.

Techniques: Expressing, Migration, Western Blot